Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Structural biology in cellulo: Minding the gap between conceptualization and realization
Δημιουργός/Συγγραφέας:	Kyrilis, Fotis L Low, Jason K K Mackay, Joel P [EL] Καστρίτης, Παναγιώτης[EN] Kastritis, Panagiotis
Ημερομηνία:	2024-08
Γλώσσα:	Αγγλικά
ISSN:	0959440X
DOI:	10.1016/j.sbi.2024.102843
Άλλο:	38788606
Περίληψη:	Recent technological advances have deepened our perception of cellular structure. However, most structural data doesn't originate from intact cells, limiting our understanding of cellular processes. Here, we discuss current and future developments that will bring us towards a structural picture of the cell. Electron cryotomography is the standard bearer, with its ability to provide in cellulo snapshots. Single-particle electron microscopy (of purified biomolecules and of complex mixtures) and covalent crosslinking combined with mass spectrometry also have significant roles to play, as do artificial intelligence algorithms in their many guises. To integrate these multiple approaches, data curation and standardisation will be critical - as is the need to expand efforts beyond our current protein-centric view to the other (macro)molecules that sustain life.
Τίτλος πηγής δημοσίευσης:	Current opinion in structural biology
Τόμος/Κεφάλαιο:	87
Θεματική Κατηγορία:	[EL] Βιοχημεία[EN] Biochemistry [EL] Δομική Βιολογία[EN] Structural Biology [EL] Φασματοσκοπία[EN] Spectroscopy [EL] Εφαρμοσμένη οπτική. Φωτονική[EN] Applied optics. Photonics
Λέξεις-Κλειδιά:	humans mass spectrometry cryoelectron microscopy
EU Grant:	Horizon Europe ERA Chair “hot4cryo”
EU Grant identifier:	101086665 03Z22HN23 03Z22HI2 03COV04 ZS/2016/04/78115 391498659 RTG 2467
Κάτοχος πνευματικών δικαιωμάτων:	© 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.
Όροι και προϋποθέσεις δικαιωμάτων:	This is an open access article under the CC BY license.
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.1016/j.sbi.2024.102843
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο